Effects of 1 MHz ultrasound on Chinese hamster V-79 cells: cavitational mechanisms and effects on proliferation

Book · Fri Jan 01 04:00:00 EST 1982

OSTI ID:5999632

Ciaravino, V

An assessment of acoustic cavitation as a primary physical mechanism in producing chemical and biological effects has been made. Chemical effects have been demonstrated through experimental protocols involving the release of iodine from sodium iodide. Biological effects have been shown by procedures assessing cell lysis and growth of in vitro Chinese hamsters V-79 cells. An important conclusion reached through these assessments is that the threshold level at which acoustic cavitation can exert an effect is dependent on the sensitivity of the experimental system being exposed. The proliferation of mitotically synchronous in vitro Chinese hamster V-79 cells exposed to 1 MHz ultrasound was investigated. Cell growth was assessed in the first three hours after sonication (3 W/cm/sup 2/ for 1 min) and was found to decrease to approx. 60 percent of control values. At an intensity of 3 W/cm/sup 2/ and exposure durations of 0.1, 1, 2, 5, and 10 min., mitotic cells underwent respectively increasing amounts of lysis. The remaining intact cells were observed for growth rate as indicated by the timed formation of colonies from single cells. The results indicated an immediate decrease in colony size (p < .01); however, colony growth rate was observed to be unaffected in sonicated cells (p > 0.05).

• OSTI ID: 5999632
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBON 14 COMPOUNDS
CELL PROLIFERATION
GROWTH
HAMSTERS
IN VITRO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
LYSIS
MAMMALS
ORGANS
RESPIRATORY SYSTEM
RODENTS
SURVIVAL TIME
TRACER TECHNIQUES
ULTRASONOGRAPHY
VERTEBRATES