Effects of abdominal irradiation on intestinal transport in the rat as assessed with isolated epithelial cells
The time course of the effect of exposure to sublethal irradiation on transport of several substrates by the intestine has been studied using isolated enterocytes. Rats received a single dose of 6 Gy to the abdomen, and isolated intestinal epithelial cells were prepared 3, 7, and 14 days later. The ability of the cells to take up D-glucose, L-leucine, and glycyl-L-leucine was assessed using 2.5-min incubation periods and was compared with the uptake in control cells. Comparison of uptake showed that D-glucose and glycly-L-leucine uptake was elevated 3 days after irradiation while that of L-leucine was unaffected. By 14 days after irradiation the glucose and glycyl-L-leucine uptake had returned to normal but the L-leucine transport was depressed. These data indicate that the effects of irradiation upon substrate transport in the intestines are not uniform and that although the cell population is initially reduced the remaining cells can compensate by increasing their transport capacity.
- Research Organization:
- Univ. of Alberta, Edmonton
- OSTI ID:
- 5998234
- Journal Information:
- Radiat. Res.; (United States), Vol. 101:1
- Country of Publication:
- United States
- Language:
- English
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CARBON 14 COMPOUNDS
MEMBRANE TRANSPORT
GLUCOSE
INTESTINES
SUBLETHAL IRRADIATION
TRANSMISSION ELECTRON MICROSCOPY
LEUCINE
TRITIUM COMPOUNDS
ABDOMEN
CESIUM 137
GAMMA RADIATION
RATS
ALDEHYDES
ALKALI METAL ISOTOPES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BODY AREAS
CARBOHYDRATES
CARBOXYLIC ACIDS
CESIUM ISOTOPES
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
GASTROINTESTINAL TRACT
HEXOSES
IONIZING RADIATIONS
IRRADIATION
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MICROSCOPY
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIATIONS
RADIOISOTOPES
RODENTS
SACCHARIDES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals
560121 - Radiation Effects on Cells- External Source- (-1987)