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Improvement of the periodate-borohydride surface-labeling method for human blood platelets

Journal Article · · Thromb. Res.; (United States)
; ;
The periodate/sodium boro(/sup 3/H)hydride ((/sup 3/H)-NaBH4) method is extensively used for the specific labeling of cell surface glycoproteins. Reduction with tritiated borohydride is also used in other surface-labeling techniques, the neuraminidase/galactose oxidase/(/sup 3/H)-NaBH4 method (specific for terminal galactose and N-acetyl-galactosamine residues) and the pyridoxal phosphate/(/sup 3/H)-NaBH4 method (specific for protein). By modification of the reaction conditions during the periodate-oxidation and borohydride-reduction, the ratio of the incorporated to the total added radioactivity could be increased by a factor of 50, while the specific activity of the labeled material was twice as high as in the original method. Alternatively, by another modification, the specific activity of the labeled material could be increased about 10-fold. The influence of the most important parameters was investigated in detail. Sodium dodecyl sulfate gel electrophoresis and fluorography demonstrate that the labeling pattern of the membrane glycoproteins is the same as with the conventional method.
Research Organization:
Theodor Kocher Institute, University of Berne, Switzerland
OSTI ID:
5994746
Journal Information:
Thromb. Res.; (United States), Journal Name: Thromb. Res.; (United States) Vol. 29:1; ISSN THBRA
Country of Publication:
United States
Language:
English

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Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
ALKALI METAL COMPOUNDS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
BOROHYDRIDES
BORON COMPOUNDS
CARBOHYDRATES
CHEMICAL PREPARATION
GLUCOPROTEINS
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
IODINE COMPOUNDS
LABELLED COMPOUNDS
LABELLING
MATERIALS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PERIODATES
PROTEINS
SACCHARIDES
SODIUM COMPOUNDS
SYNTHESIS
TRITIUM COMPOUNDS