Diacylglycerol production induced by growth hormone in Ob1771 preadipocytes arises from phosphatidylcholine breakdown
- Universite de Nice-Sophia Antipolis (France)
Growth Hormone has recently been shown to stimulate the formation of diacylglycerol in Ob1771 mouse preadipocyte cells without increasing inositol lipid turnover. Addition of growth hormone to Ob1771 cells prelabelled with ({sup 3}H)glycerol or ({sup 3}H)choline led to a rapid, transient and stoechiometric formation of labelled diacylglycerol and phosphocholine, respectively. In contrast, no change was observed in the level of choline and phosphatidic acid whereas the release of water-soluble metabolites in ({sup 3}H)ethanolamine prelabelled cells exposed to growth hormone was hardly detectable. Stimulation by growth hormone of cells prelabelled with (2-palmitoyl 9, 10 ({sup 3}H))phosphatidylcholine also induced the production of labelled diacyglycerol. Pertussis toxin abolished both diacylglycerol and phosphocholine formation induced by growth hormone. It is concluded that growth hormone mediates diacylglycerol production in Ob1771 cells by means of phosphatidylcholine breakdown involving a phospholipase C which is likely coupled to the growth hormone receptor via a pertussis toxin-sensitive G-protein.
- OSTI ID:
- 5989455
- Journal Information:
- Biochemical and Biophysical Research Communications; (USA), Vol. 173:3; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLYCEROL
BIOSYNTHESIS
PHOSPHOLIPIDS
METABOLISM
STH
BIOCHEMICAL REACTION KINETICS
ADIPOSE TISSUE
CHOLINE
FAT CELLS
LIPASES
MICE
RECEPTORS
TOXINS
TRACER TECHNIQUES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
BODY
CARBOXYLESTERASES
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DRUGS
ENZYMES
ESTERASES
ESTERS
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
QUATERNARY COMPOUNDS
REACTION KINETICS
RODENTS
SOMATIC CELLS
SYNTHESIS
TISSUES
TOXIC MATERIALS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques