Autoradiographic localization of endothelin-1 binding sites in porcine skin

Journal Article · Mon Dec 31 23:00:00 EST 1990 · Journal of Investigative Dermatology; (USA)

DOI:https://doi.org/10.1111/1523-1747.ep12515951· OSTI ID:5989349

Zhao, Y D; Springall, D R; Wharton, J; Polak, J M ^[1]

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1. Royal Postgraduate Medical School, London (England)

Autoradiographic techniques and {sup 125}I-labeled endothelin-1 were used to study the distribution of endothelin-1 binding sites in porcine skin. Specific endothelin-1 binding sites were localized to blood vessels (capillaries, deep cutaneous vascular plexus, arteries, and arterioles), the deep dermal and connective tissue sheath of hair follicles, sebaceous and sweat glands, and arrector pili muscle. Specific binding was inhibited by endothelin-2 and endothelin-3 as well as endothelin-1. Non-specific binding was found in the epidermis and the medulla of hair follicles. No binding was found in connective tissue or fat. These vascular binding sites may represent endothelin receptors, in keeping with the known cutaneous vasoconstrictor actions of the peptide. If all binding sites are receptors, the results suggest that endothelin could also regulate the function of sweat glands and may have trophic effects in the skin.

• OSTI ID: 5989349
• Journal Information: Journal of Investigative Dermatology; (USA), Journal Name: Journal of Investigative Dermatology; (USA) Vol. 96:1; ISSN 0022-202X; ISSN JIDEA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
BLOOD VESSELS
BODY
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SKIN
SWINE
TISSUE DISTRIBUTION
VASOCONSTRICTORS
VERTEBRATES