Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation

Journal Article · Wed Aug 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.15.5739· OSTI ID:5988273

Diener, D R; Curry, A M; Johnson, K A; Williams, B D; Rosenbaum, J L ^[1]; Lefebvre, P A ^[2]; Kindle, K L ^[3]

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1. Yale Univ., New Haven, CT (USA)
2. Univ. of Minnesota, St. Paul (USA)
3. Cornell Univ., Ithaca, NY (USA)

The biflagellate alga Chlamydomonas has been used extensively in the genetic and biochemical analysis of flagellar assembly and motility. The authors have restored motility to a paralyzed-flagella mutant of Chlamydomonas by transforming with the corresponding wild-type gene. A nitrate reductase-deficient paralyzed-flagella strain, nit1-305 pf-14, carrying mutations in the genes for nitrate reductase and radial spoke protein 3, was transformed with wild-type copies of both genes. Two-thirds of the cells that survived nitrate selection also regained motility, indicating that they had been transformed with both the nitrate reductase and radial spoke protein 3 genes. Transformants typically contained multiple copies of both genes, genetically linked to each other, but not linked to the original mutant loci. Complementation of paralyzed-flagella mutants by transformation is a powerful tool for investigating flagellar assembly and function.

• OSTI ID: 5988273
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:15; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOLOGY
CELL TRANSFORMATIONS
CHLAMYDOMONAS
CHLOROPHYCOTA
DNA
ELECTROPHORESIS
ENZYMES
GENETICS
MICROORGANISMS
MOBILITY
MUTANTS
NITRATES
NITROGEN COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PLANTS
TWO-DIMENSIONAL ELECTROPHORESIS
UNICELLULAR ALGAE