An effective fixative for glucocorticoid receptors in fetal tissues

Journal Article · Fri Jan 01 00:00:00 EST 1982 · J. Craniofacial Genet. Dev. Biol.; (United States)

OSTI ID:5987387

Koga, T; Kurisu, K

As a preliminary study in an autoradiographic study of glucocorticoid (GC) receptor localization in orofacial tissues of mouse fetuses, a search was made to determine the most effective fixative for preservation of the GC-receptor complex. Twelve-day-old mouse fetuses were administered tritiated triamcinolone acetonide (/sup 3/H-TAC) intraamniotically and subsequently processed by one of the following three procedures: freeze-drying, prefixation with Karnovsky's fixative, or the catechin fixative (Karnovsky's fixative containing 1% D-catechin) and postfixation with osmium tetroxide. Light microscopic autoradiography and liquid scintillation counting of the specimens revealed that the catechin fixative gave the best results for fixation of the steroid-receptor complex and preservation of tissue structure. Light and electron microscopic autoradiographic studies of the time course of the localization of /sup 3/H-TAC in palatal shelves supported the catechin fixative as being the most effective in preservation of GC-receptor or ligand complexes.

• Research Organization: Department of Oral Anatomy, Faculty of Dentistry, Kyushu University, Fukuoka, Japan
• OSTI ID: 5987387
• Journal Information: J. Craniofacial Genet. Dev. Biol.; (United States), Vol. 2:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FETUSES
AUTORADIOGRAPHY
GLUCOCORTICOIDS
RECEPTORS
BIOCHEMISTRY
MICE
PATHOLOGY
TRITIUM COMPOUNDS
ADRENAL HORMONES
ANIMALS
CHEMISTRY
CORTICOSTEROIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
PREGNANES
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques