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Mutation of a protein kinase C phosphorylation site in the erbB protein of avian erythroblastosis virus

Journal Article · · J. Virol.; (United States)
OSTI ID:5983605
; ;
Tumor promoter-stimulated phosphorylation of threonine 98 of the erbB protein of avian erythroblastosis virus (AEV) correlates with inhibition of erbB-dependent mitogenesis. To more clearly define the role of phosphorylation of this residue in regulation of the activity of the erbB protein, the authors have constructed erbB mutations which encode alanine (Ala-98), tyrosine (Tyr-98), or serine (Ser-98) at position 98. The biosynthesis and stability of the three mutant proteins were similar to those of the wild-type erbB protein, and all three retained the ability to transform chicken embryo fibroblasts. Treatment of transformed CEF with 12-tetradecanoylphorbol-13-acetate (TPA) stimulated incorporation of /sup 32/P/sub i/ into wild-type and mutant erbB proteins and resulted in a slight decrease in the electrophoretic mobilities of all the erbB proteins. Cells transformed by wild-type and mutant AEV were equally sensitive to TPA-dependent inhibition of growth in soft agar and TPA-dependent inhibition of (/sup 3/H)thymidine incorporation. These data indicate that phosphorylation of threonine 98 of the erbB protein is not responsible for TPA-dependent inhibition of growth of AEV-transformed cells or TPA-induced inhibition of erbB-dependent tyrosine phosphorylation. TPA-stimulated phosphorylation of the erbB protein at any other sites may mediate these effects. The data also show that subtle changes in a phosphorylation site drastically alter recognition by protein kinases.
Research Organization:
Rockefeller Univ., New York, NY (USA)
OSTI ID:
5983605
Journal Information:
J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:10; ISSN JOVIA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALANINES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CARCINOGENS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
ESTERS
EVEN-ODD NUCLEI
GENETIC EFFECTS
GROWTH
HYDROXY ACIDS
INHIBITION
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
METHIONINE
MUTAGENESIS
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RADIOISOTOPES
SEPARATION PROCESSES
SERINE
SULFUR 35
SULFUR ISOTOPES
THREONINE
TRANSFERASES
TYROSINE