Purification of an angiotensin II binding protein by using antibodies to a peptide encoded by angiotensin II complementary RNA

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.8.2518· OSTI ID:5983453

Elton, T S; Dion, L D; Bost, K L; Oparil, S; Blalock, J E

The authors have generated a monospecific antibody to a synthetic peptide encoded by an RNA complementary to the mRNA for angiotensin II (AII) and determined whether this antibody recognizes the AII receptor. They demonstrate that the antibody competes specifically with /sup 125/I-labeled AII for the same binding site on rat adrenal membranes. Furthermore, they show this antibody inhibits the secretion of aldosterone from cultured rat adrenal cells, suggesting that the antibody recognizes the biologically relevant AII receptor. Finally, they demonstrate that antibody to the complementary peptide can be used to immunoaffinity-purify a protein of M/sub r/ 66,000 that specifically binds radiolabeled AII.

• Research Organization: Univ. of Alabama, Birmingham (USA)
• OSTI ID: 5983453
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:8; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
AMINO ACID SEQUENCE
ANGIOTENSIN
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR AGENTS
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENDOCRINE GLANDS
GLANDS
GLOBULINS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MAMMALS
MEMBRANE PROTEINS
MESSENGER-RNA
MINERALOCORTICOIDS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
PURIFICATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RATS
RECEPTORS
RNA
RODENTS
SECRETION
STEROIDS
TRACER TECHNIQUES
VASOCONSTRICTORS
VERTEBRATES