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ADP activates protooncogene expression in renal epithelial cells

Journal Article · · Am. J. Physiol.: Heart Circ. Physiol.; (United States)
OSTI ID:5982860
; ;
Purine nucleotides, particularly adenosine diphosphate (ADP), are the most potent mitogens known for monkey kidney epithelial cells of the BSC-1 line. To explore the molecular mechanisms by which ADP exerts its mitogenic effect, the authors tested the hypothesis that stimulation of DNA synthesis in these renal cells is mediated by activation of protooncogenes. Transcripts of the c-Ha-ras protooncogene were identified in quiescent, high density cells. Addition of ADP to the culture medium stimulated protooncogene expression fourfold. Maximal expression of c-ras was observed by 12 h after exposure to ADP, and preceded the initiation of DNA synthesis. Expression of the c-myc protooncogene was not detected in unstimulated cells, but accumulated maximally after 1 h of exposure to ADP. As with ADP-stimulated expression of the c-ras protooncogene, transcripts of the transferrin receptor gene reached a maximal value at 12 h, whereas the abundance of ..gamma..-actin mRNA was not altered for up to 24 h. The results indicate that exogenous ADP stimulated protooncogene expression before initiation of DNA synthesis in renal epithelial cells in culture. These findings suggest that some physiological effect of this adenine nucleotide could be mediated by proteins specified by protooncogenes.
Research Organization:
Univ. of Chicago, IL (USA)
OSTI ID:
5982860
Journal Information:
Am. J. Physiol.: Heart Circ. Physiol.; (United States), Journal Name: Am. J. Physiol.: Heart Circ. Physiol.; (United States) Vol. 21:6; ISSN AJPPD
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
ANIMAL CELLS
ANIMAL TISSUES
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CHEMICAL ACTIVATION
CHEMISTRY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
DNA REPLICATION
EPITHELIUM
FIBROBLASTS
GENES
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
IN VITRO
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIGHT NUCLEI
MESSENGER-RNA
MITOGENS
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ONCOGENES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RNA
SOMATIC CELLS
THYMIDINE
TISSUES
TRANSCRIPTION
TRITIUM COMPOUNDS