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Use of a gamma- or hard beta-emitting radioisotope to assess milk intake in suckling mouse pups

Journal Article · · J. Nutr.; (United States)
OSTI ID:5977150
A radioisotopic method to assess milk intake during suckling in mice is described. The method is based on the transfer of a gamma- or hard beta-emitting radioisotope from the lactating mother to the litter. The radioactivity of individual pups is determined at intervals by whole-body counting. The transfer of /sup 22/Na, /sup 32/P as orthophosphate, /sup 51/Cr as chromate, and /sup 125/I as iodide was examined; /sup 125/I showed particularly suitable characteristics. The procedure proved to be simple, accurate and reproducible for the comparison of mild consumption between pups within a litter and between litters. The procedure was suitable for studies using a foster mother.
Research Organization:
Department of Biological Sciences, University of Aston, Birmingham, UK
OSTI ID:
5977150
Journal Information:
J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 113:4; ISSN JONUA
Country of Publication:
United States
Language:
English