Separation and detection of chloramphenicol with its nitro and amino analogues using HPLC equipped with reductive/oxidative electrochemical and ultraviolet detectors

Conference · Fri May 01 04:00:00 UTC 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5976893

Abou-Khalil, S; Abou-Khalil, W H; Masoud, A N; Yunis, A A

In their studies on chloramphenicol (CAP)-associated bone marrow injury, they have postulated that the p-nitro group of CAP undergoes nitro reduction yielding toxic and highly reactive derivatives. Elucidating the pathogenetic mechanism in CAP-induced injury requires specific and sensitive analytical tool to detect CAP metabolites that may be produced by mammalian cells. To accomplish their objective they have installed a uniquely suited HPLC system equipped with simultaneous dual detection: ultraviolet (UV) and electrochemical (EC). The system was constructed and specially designed to accommodate the use of the EC detector in both reductive and oxidative modes. Using this system, separation, quantitation (picomoles range) and identification of CAP were achieved with the following nitro and amino analogues: dehydrochloramphenicol, nitrophenylaminopropanedione, nitroso-chloramphenicol and aminochloramphenicol. Retention characteristics, hydrodynamic voltammograms under reductive and oxidative conditions and UV absorbance were determined. Applicability of the procedure to biological fluids was demonstrated by separation and detection of CAP after incubation with human blood. The data demonstrated that the reductive/oxidative EC modes in tandem with the conventional UV detector increased exponentially the selectivity and sensitivity of the system, and also provided a powerful basis for the identification of unknown metabolites.

• Research Organization: Univ. of Miami School of Medicine, FL
• OSTI ID: 5976893
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISTRY
CHLORAMPHENICOL
CHROMATOGRAPHY
DRUGS
ELECTROCHEMISTRY
ELECTROMAGNETIC RADIATION
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDATION
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONS
REDUCTION
SEPARATION PROCESSES
SPECTRA
TOXICITY
ULTRAVIOLET RADIATION
ULTRAVIOLET SPECTRA