Sister chromatid exchanges and chromosome aberrations induced by radiosensitizing agents in bone marrow cells of treated tumor-bearing mice
The frequency of sister chromatid exchanges (SCE) in vivo and chromosome aberrations and/or alterations were analyzed from the bone marrow cells of the treated dbrB tumor-bearing DBA/1J inbred mouse host. The results were compared with analogous data obtained from the bone marrow cells of untreated tumor-bearing mice for evaluation of the ''indirect,'' i.e., somatic stress, effect on the normal host cells following triple-agent therapy intended for a mammary adenocarcinoma. Misonidazole (MIS), which is a known radiosensitizing drug, microwave hyperthermia (delta), and X-radiation (X) were used as therapeutic agents. Significant (P less than 0.05) numbers of SCE were induced in the bone marrow cells of the mice whose tumors received these triple-agent treatments (MIS + delta + X) simultaneously as compared with values of SCE per cell noted in bone marrow cells of untreated tumor-bearing control mice. The highest number of chromosome aberrations and alterations, including an increase in heteroploidy, was also noticed in the bone marrow cells of the mice whose tumors were treated simultaneously with MIS + delta + X. The triple-agent therapy on dbrB tumor also resulted in an unusually high polyploid metaphase plate in the bone marrow cell consisting of 320 chromosomes, indicating that this mode of therapy may act directly on the genetic material of the tumor-bearing host cells, inducing cytogenetic abnormalities as a side effect.
- Research Organization:
- Department of Biology, New York University, NY
- OSTI ID:
- 5975342
- Journal Information:
- JNCI, J. Natl. Cancer Inst.; (United States), Journal Name: JNCI, J. Natl. Cancer Inst.; (United States) Vol. 70:3; ISSN JJIND
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTINEOPLASTIC DRUGS
AZOLES
BIOLOGICAL EFFECTS
BODY
BODY TEMPERATURE
BONE MARROW CELLS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
GLANDS
HETEROCYCLIC COMPOUNDS
HYPERTHERMIA
IMIDAZOLES
IONIZING RADIATIONS
MAMMALS
MAMMARY GLANDS
MEDICINE
MICE
MISONIDAZOLE
MUTATIONS
NEOPLASMS
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIATIONS
RADIOLOGY
RADIOSENSITIZERS
RADIOTHERAPY
RODENTS
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
SYNERGISM
THERAPY
VERTEBRATES
X RADIATION