Interlaboratory collaborative study on sister chromatid exchange induction in vivo
Nine laboratories participated in a comparative analysis of the ability of BP/PYR and 2AAF/4AAF to induce sister chromatid exchanges (SCEs) in vivo, using mice, rats and Chinese hamsters. Bone marrow, intestinal epithelium and peripheral blood lymphocytes were used as target tissues, following either gavage or intraperitoneal (ip) administration of the test chemical. Based on a statistical analysis of the SCE data by ANOVA and using an ..cap alpha.. of 0.05, BP induced a highly significant increase in SCEs in every tissue sampled in each species and among all strains of mice by both routes. Treatment with PYR induced a marginally positive SCE response in 5 of 15 experiments (considering male and female responses separately). By both routes, treatment with 2AAF induced a highly significant increase in SCE frequency in bone marrow cells of all mouse strains, in bone marrow and intestinal epithelial cells of Chinese hamsters. Treatment with 4AAF indiced a marginally positive SCE response in 6 of 15 experiments (considering male and female responses separately). The 2 carcinogens, BP and 2AAF, were clearly active regardless of species, strain, sex, tissue and route of administration and more potent than either of the two purportedly non-carcinogenic structural analogues, PYR and 4AAF, respectively. 40 refs., 10 figs., 19 tabs.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 5488193
- Report Number(s):
- BNL-38070; CONF-8503244-1; ON: DE86011204
- Resource Relation:
- Conference: Evaluation of short-term tests for carcinogens meeting, Cap D' Adge, France, 13 Mar 1985; Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACETYLAMINOFLUORENES
MUTAGENESIS
BENZOPYRENE
PYRENE
BONE MARROW CELLS
CRYPT CELLS
EXPERIMENTAL DATA
HAMSTERS
LYMPHOCYTES
MICE
RATS
SISTER CHROMATID EXCHANGES
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DATA
HYDROCARBONS
INFORMATION
LEUKOCYTES
MAMMALS
MATERIALS
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
RODENTS
SOMATIC CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)