Lectins discriminate between pathogenic and nonpathogenic South American trypanosomes
Cell surface carbohydrates of Trypanosoma cruzi, Trypanosoma rangeli, and Trypanosoma conorhini were analyzed by a micro-agglutination assay employing 27 highly purified lectins and by binding assays using various /sup 125/I-labeled lectins. The following seven lectins discriminated between the trypanosomes: 1) tomato lectin (an N-acetyl-D-glucosamine-binding protein), both in purified form and as crude tomato juice; 2) Bauhinea purpurea and Sophora japonica lectins (both N-acetyl-D-galactosamine-binding proteins), which selectively agglutinated T. cruzi; 3) Vicia villosa (an N-acetyl-D-galactosamine-binding protein) which was specific for T. rangeli; 4) peanut lectin (a D-galactose-binding protein) both in purified form and as crude saline extract; and 5) Ulex europaeus and Lotus tetragonolobus (both L-fucose-binding proteins) lectins which reacted only with T. conorhini. Binding studies with 125I-labeled lectins were performed to find whether unagglutinated cells of the three different species of trypanosomes might have receptors for these lectins, in which case absence of agglutination could be due to a peculiar arrangement of the receptors. These assays essentially confirmed the agglutination experiments.
- Research Organization:
- Tufts Univ. School of Medicine, Boston, MA
- OSTI ID:
- 5973718
- Journal Information:
- Am. J. Trop. Med. Hyg.; (United States), Journal Name: Am. J. Trop. Med. Hyg.; (United States) Vol. 33:5; ISSN AJTHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOASSAY
CARBOHYDRATES
CHEMICAL BONDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LECTINS
MASTIGOPHORA
MICROANALYSIS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
RADIOISOTOPES
TRACER TECHNIQUES
TRYPANOSOMA