Metabolic engineering of Klebsiella oxytoca M5A1 for ethanol production from xylose and glucose

Journal Article · Tue Oct 01 04:00:00 EDT 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5965059

Ohta, Kazuyoshi; Beall, D S; Mejia, J P; Shanmugam, K T; Ingram, L O ^[1]

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1. Univ. of Florida, Gainesville (United States)

The efficient diversion of pyruvate from normal fermentative pathways to ethanol production in Klebsiella oxytoca M5A1 requires the expression of Zymomanas mobilis genes encoding both pyruvate decarboxylase and alcohol dehydrogenase. Final ethanol concentrations obtained with the best recombinant, strain M5A1 (pLOI555), were in excess of 40 g/liter with an efficiency of 0.48 g of ethanol (xylose) and 0.50 g of ethanol (glucose) per g of sugar, as compared with a theoretical maximum of 0.51 of ethanol per g of sugar. The maximal volumetric productivity per hour for both sugars was 2.0 g/liter. This volumetric productivity with xylose is almost twice that previously obtained with ethanologenic Escherichia coli. Succinate was also produced as a minor product during fermentation.

• OSTI ID: 5965059
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 57:10; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
BIOTECHNOLOGY
CARBOHYDRATES
CARBON-CARBON LYASES
CARBOXY-LYASES
DECARBOXYLASES
ENZYMES
ETHANOL
FERMENTATION
GENETIC ENGINEERING
GLUCOSE
HEXOSES
HYDROXY COMPOUNDS
KLEBSIELLA
LYASES
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PENTOSES
PRODUCTIVITY
PROTEINS
SACCHARIDES
SYNTHESIS
XYLOSE
ZYMOMONAS MOBILIS