Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Applied and Environmental Microbiology; (USA)

OSTI ID:5105184

Alterthum, F ^[1]; Ingram, L O ^[2]

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1. Univ. de Sao Paulo (Brazil)
2. Univ. of Florida, Gainesville (USA)

Lactose and all of the major sugars (glucose, xylose, arabinose, galactose, and mannose) present in cellulose and hemicellulose were converted to ethanol by recombinant Escherichia coli containing plasmid-borne genes encoding the enzymes for the ethanol pathway from Zymomonas mobilis. Environmental tolerances, plasmid stability, expression of Z. mobilis pyruvate decarboxylase, substrate range, and ethanol production (from glucose, lactose, and xylose) were compared among eight American Type Culture Collection strains. E. coli ATCC 9637(pLOI297), ATCC 11303(pLOI297), and ATCC 15224(pLOI297) were selected for further development on the basis of environmental hardiness and ethanol production. Volumetric ethanol productivities per hour in batch culture were 1.4 g/liter for glucose (12%), 1.3 g/liter for lactose (12%), and 0.64 g/liter for xylose (8%). Ethanol productivities per hour ranged from 2.1 g/g of cell dry weight with 12% glucose to 1.3 g/g of cell dry weight with 8% xylose. The ethanol yield per gram of xylose was higher for recombinant E. coli than commonly reported for Saccharomyces cerevisiae with glucose. Glucose (12%), lactose (12%), and xylose (8%) were converted to (by volume) 7.2% ethanol, 6.5% ethanol, and 5.2% ethanol, respectively.

• OSTI ID: 5105184
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 55:8; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
CARBOHYDRATES
CELL CONSTITUENTS
COMPARATIVE EVALUATIONS
DISACCHARIDES
DNA
ESCHERICHIA COLI
ETHANOL
FERMENTATION
GENETIC ENGINEERING
GLUCOSE
HEXOSES
HYDROXY COMPOUNDS
LACTOSE
MICROORGANISMS
MONOSACCHARIDES
NUCLEIC ACIDS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
PLASMIDS
PRODUCTIVITY
RECOMBINANT DNA
SACCHARIDES
SYNTHESIS
XYLOSE
ZYMOMONAS MOBILIS