Yellow affinity substance involved in the cellulolytic system of Clostridium thermocellum

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Curr. Microbiol.; (United States)

DOI:https://doi.org/10.1007/BF01567580· OSTI ID:5964720

Ljungdahl, L G; Pettersson, B; Eriksson, K E; Wiegel, J

Clostridium thermocellum produces a yellow substance when fermenting cellulose. This substance is attached to the cellulose particles. Cellulose with the yellow substance, obtained from cultures of C. thermocellum, binds effectively endo-1,4-..beta..-glucanase produced by the bacterium and was used in an affinity column for purification of the enzyme. At the beginning of fermentation of cellulose, most of the endoglucanase was bound to the yellow cellulose. As the fermentation proceeded, the enzyme appeared free in the culture fluid. The endoglucanase bound to the yellow cellulose could be extracted by distilled water from the cellulose, but not by solutions with 5 mM or higher concentrations of salts or buffers. It is proposed that the yellow substance is involved in the cellulolytic system of C. thermocellum. 30 references, 3 figures, 3 tables.

• Research Organization: Swedish Forest Products Research Lab., Stockholm
• DOE Contract Number: AS09-79ER10499
• OSTI ID: 5964720
• Journal Information: Curr. Microbiol.; (United States), Journal Name: Curr. Microbiol.; (United States) Vol. 9; ISSN CUMID
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
BACTERIA
BIOCONVERSION
CARBOHYDRATES
CELLULASE
CELLULOLYTIC ACTIVITY
CELLULOSE
CHEMICAL BONDS
CLOSTRIDIUM
CLOSTRIDIUM THERMOCELLUM
DATA
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FERMENTATION
GLYCOSYL HYDROLASES
HYDROLASES
INFORMATION
MICROORGANISMS
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
PURIFICATION
SACCHARIDES