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Composition of the cellulase complex of clostridium thermocellum

Journal Article · · Biochemistry (Engl. Transl.); (United States)
OSTI ID:5965678
The anaerobic thermophilic cellulolytic bacterium C. thermocellum is considered as a potential organism for the industrial direct bioconversion of cellulose to ethanol. The activities and localization of various cellulolytic enzymes in C. thermocellum are determined here. Preparative methods and determination of enzymes are discussed. Analytical methods are examined. The authors determine that C. thermocellum has all six known cellulolytic enzymes, and that in certain cases up to 50% of the intracellular activity of the indicated enzymes is localized outside the cells.
Research Organization:
Institute of the Biochemistry and Physiology of Microorganisms, Academy of Sciences of the USSR, Pushchino
OSTI ID:
5965678
Journal Information:
Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:1; ISSN BIORA
Country of Publication:
United States
Language:
English