Coordinate regulation of stromelysin and collagenase genes determined with cDNA probes

Journal Article · Fri May 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.9.2600· OSTI ID:5964608

Frisch, S M; Clark, E J; Werb, Z

Secreted proteinases are required for tumor metastasis, angiogenesis, and tissue remodeling during wound healing and embryonic growth. Thus, the regulation of the genes of secreted proteinases may serve as an interesting model for growth-controlled genes in general. The authors studied the genes of the secreted proteinases stromelysin and collagenase by using molecularly cloned cDNAs from each proteinase. Stromelysin cDNA was cloned by differential screening of a total cDNA library from rabbit synovial cells treated with phorbol 12-myristate 13-acetate, which yielded a clone of 1.2 kilobase pairs; collagenase cDNA was obtained by cloning reverse transcripts of anti-collagenase-immunoadsorbed polysomal mRNA, which yielded a clone of 0.8 kilobase pairs. Stromelysin and collagenase mRNA species of 2.2 and 2.4 kilobases, respectively, were detected on hybridization blots of RNA from phorbol 12-myristate 13-acetate-treated but not untreated rabbit synovial cells. Expression of stromelysin mRNA was also induced in rabbit alveolar macrophages and rabbit brain capillary endothelial cells treated with phorbol 12-myristate 13-acetate. Stromelysin and collagenase mRNA were both induced by phorbol 12-myristate 13-acetate and cytochalasin B at a constant ratio of the two gene products; this suggest coordinate regulation. The fact that induction was blocked after inhibition of protein synthesis by cycloheximide implicates an indirect signal transduction pathway that requires new protein synthesis.

• Research Organization: Univ. of California, San Francisco
• DOE Contract Number: AC03-76SF01012
• OSTI ID: 5964608
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:9; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
CARCINOGENS
COLLAGEN
DAYS LIVING RADIOISOTOPES
DNA
ELECTROPHORESIS
ENDOTHELIUM
ENZYMES
ESTERS
GENE REGULATION
GENES
HEALING
HYBRIDIZATION
HYDROLASES
INJURIES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MESSENGER-RNA
METASTASES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RABBITS
RADIOISOTOPES
RECOMBINANT DNA
RECOVERY
RNA
SCLEROPROTEINS
TISSUES
VERTEBRATES
WOUNDS