An unusual form of lipid linkage to the CD45 peptide

Journal Article · Fri Nov 02 04:00:00 EST 1990 · Science (Washington, D.C.); (USA)

DOI:https://doi.org/10.1126/science.2146743· OSTI ID:5962909

Takeda, Akiko; Maizel, A L ^[1]

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1. Brown Univ., Providence, RI (USA)

Some protein kinases and phosphatases are myristoylated on their amino terminus, which perhaps contributes to subcellular localization or regulation. Glycoprotein CD45, a hematopoietic tyrosine phosphatase, was examined for fatty acid content. The CD45 protein incorporated ({sup 3}H)myristate, but little ({sup 3}H)palmitate. The label was not metabolized and reincorporated into amino acids or saccharides, as revealed by peptide maps of CD45 labeled with ({sup 3}H)myristate, {sup 14}C-labeled amino acids, ({sup 35}S)methionine, or {sup 125}I, and glycosidase treatments, respectively. The myristate label was resistant to mild alkaline methanolysis and was found in fatty acid and sphingosine, indicating an unusual form of lipid attachment to CD45.

• OSTI ID: 5962909
• Journal Information: Science (Washington, D.C.); (USA), Journal Name: Science (Washington, D.C.); (USA) Vol. 250:4981; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLYCOPROTEINS
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PROTEINS
RADIOISOTOPES
SUBCELLULAR DISTRIBUTION
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TRITIUM COMPOUNDS