Protein fatty acid acylation: enzymatic synthesis of an N-myristoylglycyl peptide
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
Incubation of Saccharomyces cerevisiae strain JR153 with either (/sup 3/H)myristate or (/sup 3/H)palmitate demonstrates the synthesis of proteins that contain covalently bound fatty acids. A unique set of proteins is labeled by each fatty acid. Detailed analysis of a 20-kDa protein labeled with myristic acid demonstrates that myristate is linked to the amino-terminal glycine. We describe an enzymatic activity in yeast that will transfer myristic acid to the amino terminus of the octapeptide Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg, whose sequence was derived from a known N-myristoylated acyl protein, the catalytic subunit of cAMP-dependent protein kinase of bovine cardiac muscle. The acylation reaction is dependent on ATP and CoA, is enriched in a crude membrane fraction, and will use myristate but not palmitate as the acyl donor. Specificity of the glycyl peptide substrate is demonstrated by the observation that other glycyl peptides do not competitively inhibit myristoylation of Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg.
- Research Organization:
- Washington Univ. School of Medicine, St. Louis, MO
- OSTI ID:
- 5578911
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 9; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
AMINO ACID SEQUENCE
AMINO ACIDS
AMP
ATP
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
COVALENCE
ENZYMES
FUNGI
GLYCINE
HEXADECANOIC ACID
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGASES
MEMBRANES
MICROORGANISMS
MOLECULAR STRUCTURE
MONOCARBOXYLIC ACIDS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PLANTS
PROTEINS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
YEASTS
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
AMINO ACID SEQUENCE
AMINO ACIDS
AMP
ATP
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
COVALENCE
ENZYMES
FUNGI
GLYCINE
HEXADECANOIC ACID
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGASES
MEMBRANES
MICROORGANISMS
MOLECULAR STRUCTURE
MONOCARBOXYLIC ACIDS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PLANTS
PROTEINS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
YEASTS