A combustion method for the simultaneous determination of sup 3 H, sup 14 C, and sup 35 S in triply labeled organic samples by liquid scintillation counting

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Analytical Biochemistry; (USA)

DOI:https://doi.org/10.1016/0003-2697(90)90193-D· OSTI ID:5962725

Saito, K; Miyatake, H; Kurihara, N ^[1]

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1. Kyoto Univ. (Japan)

The organic samples triply labeled with {sup 3}H, {sup 14}C, and {sup 35}S were combusted in a flow-type combustion flask under a continuous oxygen flow. The oxygen gas containing the combustion products, which were mainly {sup 3}H{sub 2}O, {sup 14}CO{sub 2}, and {sup 35}SO{sub 2}, was introduced into successive 0.5 M H{sub 2}O{sub 2} and Oxisorb (2-methoxyethylamine). The {sup 35}SO{sub 2} and {sup 3}H{sub 2}O that had been partially adsorbed onto the inner wall of the combustion flask were recovered by rinsing the wall with an H{sub 2}O{sub 2} solution. {sup 3}H and {sup 35}S were quantitatively recovered from the H{sub 2}O{sub 2} solutions. 14C was not found in the H{sub 2}O{sub 2} solutions and was recovered from Oxisorb quantitatively. The amounts of {sup 3}H, {sup 35}S, and {sup 14}C were determined by measuring the radioactivity of the H{sub 2}O{sub 2} solutions and of Oxisorb with a liquid scintillation counter. Thus the separate determination of {sup 14}C and {sup 35}S in a sample was achieved. Through this combustion method, the materials including fresh animal tissues up to ca. 300 mg were conveniently combusted with quantitative recovery of {sup 3}H, {sup 14}C, and {sup 35}S.

• OSTI ID: 5962725
• Journal Information: Analytical Biochemistry; (USA), Journal Name: Analytical Biochemistry; (USA) Vol. 190:2; ISSN ANBCA; ISSN 0003-2697
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL REACTIONS
COMBUSTION
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
ORGANIC MATTER
OXIDATION
OXIDES
OXYGEN COMPOUNDS
PEROXIDES
RADIOISOTOPES
SCINTILLATION COUNTING
SULFUR 35
SULFUR COMPOUNDS
SULFUR DIOXIDE
SULFUR ISOTOPES
SULFUR OXIDES
THERMOCHEMICAL PROCESSES
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS