In vivo effect of human granulocyte-macrophage colony-stimulating factor on megakaryocytopoiesis

Journal Article · Fri Mar 15 00:00:00 EST 1991 · Blood (Journal of Hematology); (USA)

OSTI ID:5962410

Aglietta, M; Monzeglio, C; Sanavio, F; Apra, F; Morelli, S; Stacchini, A; Piacibello, W; Bussolino, F; Bagnara, G; Zauli, G ^[1]

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1. Universita di Torino (Italy)

The effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on megakaryocytopoiesis and platelet production was investigated in patients with normal hematopoiesis. Three findings indicated that GM-CSF plays a role in megakaryocytopoiesis. During treatment with GM-CSF (recombinant mammalian, glycosylated; Sandoz/Schering-Plough, 5.5 micrograms protein/kg/d, subcutaneously for 3 days) the percentage of megakaryocyte progenitors (megakaryocyte colony forming unit (CFU-Mk)) in S phase (evaluated by the suicide technique with high 3H-Tdr doses) increased from 31% +/- 16% to 88% +/- 11%; and the maturation profile of megakaryocytes was modified, with a relative increase in more immature stage I-III forms. Moreover, by autoradiography (after incubation of marrow cells with 125I-labeled GM-CSF) specific GM-CSF receptors were detectable on megakaryocytes. Nevertheless, the proliferative stimulus induced on the progenitors was not accompanied by enhanced platelet production (by contrast with the marked granulomonocytosis). It may be suggested that other cytokines are involved in the regulation of the intermediate and terminal stages of megakaryocytopoiesis in vivo and that their intervention is an essential prerequisite to turn the GM-CSF-induced proliferative stimulus into enhanced platelet production.

• OSTI ID: 5962410
• Journal Information: Blood (Journal of Hematology); (USA), Vol. 77:6; ISSN 0006-4971
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
BIOSYNTHESIS
ERYTHROCYTES
CELL PROLIFERATION
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
AUTORADIOGRAPHY
COLONY FORMATION
HEMATOLOGY
IODINE 125
MACROPHAGES
STEM CELLS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MATERIALS
MEDICINE
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SOMATIC CELLS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques