Biosynthetic origin of gibberellins A sub 3 and A sub 7 in cell-free preparations from seeds of Marah macrocarpus and Malus domestica

Journal Article · Sat Sep 01 04:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.1.132· OSTI ID:5962152

Albone, K S; Gaskin, P; MacMillan, J; Willis, C L ^[1]; Phinney, B O ^[2]

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1. Univ. of Bristol (England)
2. Univ. of California, Los Angeles (USA)

Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A{sub 9} (GA{sub 9}) and 2,3-dehydroGA{sub 9} to GA{sub 7}; GA{sub 9} was also metabolized to GA{sub 4} in a branch pathway. The preparation from Marah seeds also metabolized GA{sub 5} to GA{sub 3} in high yield; GA{sub 6} was a minor product and was not metabolized to GA{sub 3}. Using substrates stereospecifically labeled with deuterium, it was shown that the metabolism of GA{sub 5} to GA{sub 3} and of 2,3-dehydroGA{sub 9} to GA{sub 7} occurs with the loss of the 1{beta}-hydrogen. In cultures of Gibberella fujikuroi, mutant B1-41a, (1{beta},2{beta}-{sup 2}H{sub 2})GA{sub 4}, was metabolized to (1,2-{sup 2}H{sub 2})GA{sub 3} with the loss of the 1{alpha}- and 2{alpha}-hydrogens. These results provide further evidence that the biosynthetic origin of GA{sub 3} and GA{sub 7} in higher plants is different from that in the fungus Gibberella fujikuroi.

• OSTI ID: 5962152
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 94:1; ISSN PLPHA; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
APPLES
BIOSYNTHESIS
CARBOXYLIC ACIDS
DEUTERIUM
ESTERS
FOOD
FRUIT TREES
FRUITS
FUNGI
GIBBERELLIC ACID
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY ACIDS
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LACTONES
LIGHT NUCLEI
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SEEDS
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TREES