In vivo gibberellin biosynthesis in endosperm of Sechium edule Sw. seeds

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.93.3.1032· OSTI ID:5957546

Ceccarelli, N; Lorenzi, R ^[1]

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1. Universita degli Studi, Pisa (Italy)

Biosynthesis of gibberellins (GAs) was studied in vivo in endosperms of Sechium edule Sw. Exogenous ent-({sup 14}C)kaurene was metabolized into four major products: GA{sub 12}, GA{sub 4}, GA{sub 7} and 16,17-dihydro-16-hydroxy-GA{sub 15} alcohol glucoside. Other minor metabolites were also observed including ent-kaurenol and ent-kaurenal. Conversion of ent-({sup 14}C)kaurene to ent-karuenol glucoside by endosperm cell-free preparations in the presence of UDPG was observed. However, the finding was not confirmed in in vivo studies and is probably artifactual. Overall evidence coming from the analysis of endogenous GAs and in vitro and in vivo biosynthetic studies are discussed in relation to the possible existence in the Sechium seeds of a different route, along with the known pathway, branching from ent-kaurene or ent-7-{alpha}-hydroxykaurenoic acid and this also leading to biologically active GAs.

• OSTI ID: 5957546
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 93:3; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
ENDOSPERM
ESTERS
GIBBERELLIC ACID
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTONES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHYSIOLOGY
PLANT TISSUES
SEEDS
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TUBERS