Regulation of collagen production in freshly isolated cell populations from normal and cirrhotic rat liver: Effect of lactate

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Hepatology (Baltimore); (USA)

DOI:https://doi.org/10.1002/hep.1840130324· OSTI ID:5961745

Cerbon-Ambriz, J; Cerbon-Solorzano, J; Rojkind, M ^[1]

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1. Centro de Investigacion y de Estudios Avanzados del IPN, Mexico City (Mexico)

Previous work has shown that lactic acid, and to a lesser extent pyruvic acid, is able to increase collagen synthesis significantly in liver slices of CCl4-treated rats but not normal rats. The purpose of this report is to document which cells in the cirrhotic liver are responsible for the lactate-stimulated increase in collagen synthesis. It was found that (a) incorporation of 3H-proline into protein-bound 3H-hydroxyproline is increased threefold to fourfold in hepatocytes from CCl4-treated rats as compared with normal rat hepatocytes; (b) neither the hepatocytes from normal nor those from CCl4-treated rats modify their collagen synthesizing capacity when 30 mmol/L lactic acid was added to the incubation medium; (c) nonparenchymal cells obtained from livers of CCl4-treated rats synthesize much less collagen than hepatocytes, but their synthesis is stimulated twofold by lactic acid; (d) from the different nonparenchymal cells, only fat-storing (Ito) cells increase collagen synthesis when lactic acid is present in the incubation medium. These results suggest that the increased lactic acid levels observed in patients with alcoholic hepatic cirrhosis may play an important role in the development of fibrosis by stimulating collagen production by fat-storing (Ito) cells.

• OSTI ID: 5961745
• Journal Information: Hepatology (Baltimore); (USA), Journal Name: Hepatology (Baltimore); (USA) Vol. 13:3; ISSN 0270-9139; ISSN HPTLD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON TETRACHLORIDE
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHLORINATED ALIPHATIC HYDROCARBONS
COLLAGEN
DIGESTIVE SYSTEM DISEASES
DISEASES
HALOGENATED ALIPHATIC HYDROCARBONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LACTATES
LIVER CELLS
LIVER CIRRHOSIS
MAMMALS
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOGENESIS
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RATS
REACTION KINETICS
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES