Endotoxin reduces specific pulmonary uptake of radiolabeled monoclonal antibody to angiotensin-converting enzyme

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Journal of Nuclear Medicine; (USA)

OSTI ID:5960009

Muzykantov, V R; Puchnina, E A; Atochina, E N; Hiemish, H; Slinkin, M A; Meertsuk, F E; Danilov, S M ^[1]

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1. USSR Cardiology Research Center, Moscow (USSR)

The biodistribution of radiolabeled monoclonal antibody (Mab) to angiotensin-converting enzyme (ACE) was examined in normal and endotoxin-treated rats. Endotoxin administration at a dose of 4 mg/kg induced mild or middle pulmonary edema. The ACE activity in lung homogenate remained virtually unchanged, while the activity of serum ACE increased 15 hr after endotoxin infusion. In normal rats, anti-ACE Mab accumulates specifically in the lung after i.v. injection. Endotoxin injection induces reduction of specific pulmonary uptake of this antibody. Even in non-edematous endotoxemia, the accumulation of anti-ACE Mab antibody (Mab 9B9) decreased from 19.02 to 11.91% of ID/g of tissue without any change in accumulation of control nonspecific IgG. The antibody distribution in other organs and its blood level were almost the same as in the control. In a case of endotoxemia accompanied by increased microvascular permeability, the lung accumulation of Mab 9B9 was reduced to 9.17% of ID/g of tissue, while the accumulation of nonspecific IgG increased to 1.44% versus 0.89% in the control.

• OSTI ID: 5960009
• Journal Information: Journal of Nuclear Medicine; (USA), Vol. 32:3; ISSN 0161-5505
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
LUNGS
EDEMA
MONOCLONAL ANTIBODIES
TISSUE DISTRIBUTION
PEPTIDE HYDROLASES
ENZYME ACTIVITY
TOXINS
BIOLOGICAL EFFECTS
IMMUNOGLOBULINS
INDIUM ISOTOPES
IODINE ISOTOPES
MICE
RATS
TRACER TECHNIQUES
ANIMALS
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ANTIGENS
BODY
DISTRIBUTION
ENZYMES
GLOBULINS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROTEINS
RESPIRATORY SYSTEM
RODENTS
SYMPTOMS
TOXIC MATERIALS
VERTEBRATES
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