Identification and quantificaton of endogenous gibberellins in the vegetative shoots of normal, d1, d2, d3, and d5 Zea mays
Conference
·
· Plant Physiol., Suppl.; (United States)
OSTI ID:5957083
The authors now have determined the levels of seven endogenous GAs previously identified as native to vegetative shoots of maize. These gibberellins, GA/sub 1/, GA/sub 8/, GA/sub 19/, GA/sub 20/, GA/sub 29/, GA/sub 44/ and GA/sub 53/ are members of the early-13-hydroxylation pathway which leads to the biologically active gibberellin, GA/sub 1/. The quantification data come from the use of (/sup 13/C, /sup 3/H)-, (/sup 2/H)- or (/sup 14/C)-labeled GAs, added as internal standards to the aqueous residues. Capillary GC-MS was used to compare the peak intensities of the labeled internal standards to those of the endogenous GA(s). Gibberellin levels in the Normals ranged from 425 ng/100 g f.w. (GA/sub 19/) to 12 ng/100 g f.w. (GA/sub 1/ and GA/sub 29/). Essentially no Gas (i.e. less than 0.2 ng/100 g f.w.) were detected in the d2, d3 and d5 mutants (mutants that block steps prior to GA/sub 53/). The d1 mutant (blocked between GA/sub 20/ and GA/sub 1/) was found to have 3% the level of the GA/sub 1/ found in Normals. Interestingly, GA/sub 20/ accumulated in the d1 mutant to a level over ten times that of the Normals.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 5957083
- Report Number(s):
- CONF-8707108-
- Conference Information:
- Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Further identification of endogenous gibberellins in the shoots of pea, line G2
Journal Article
·
Fri Mar 31 23:00:00 EST 1989
· Plant Physiology, Supplement; (USA)
·
OSTI ID:6873600
Gibberellins and gravitropism in maize shoots: endogenous gibberellin-like substances and movement and metabolism of (/sup 3/)gibberellin A
Journal Article
·
Sat Feb 28 23:00:00 EST 1987
· Plant Physiol.; (United States)
·
OSTI ID:6438445
Further identification of endogenous gibberellins in the shoots of pea, line G2
Journal Article
·
Thu Nov 30 23:00:00 EST 1989
· Plant Physiology; (USA)
·
OSTI ID:5858254
Related Subjects
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON 13
CARBON 14 COMPOUNDS
CARBON ISOTOPES
CEREALS
CHROMATOGRAPHY
DEUTERIUM COMPOUNDS
EVEN-ODD NUCLEI
GAS CHROMATOGRAPHY
GRASS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAIZE
MASS SPECTROSCOPY
METABOLISM
MUTANTS
NUCLEI
PLANT GROWTH REGULATORS
PLANT STEMS
PLANTS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON 13
CARBON 14 COMPOUNDS
CARBON ISOTOPES
CEREALS
CHROMATOGRAPHY
DEUTERIUM COMPOUNDS
EVEN-ODD NUCLEI
GAS CHROMATOGRAPHY
GRASS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAIZE
MASS SPECTROSCOPY
METABOLISM
MUTANTS
NUCLEI
PLANT GROWTH REGULATORS
PLANT STEMS
PLANTS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS