Effect of caffeine on the expression of a major X-ray induced protein in human tumor cells

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3578116· OSTI ID:5956563

Hughes, E N; Boothman, D A ^[1]

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1. Univ. of Pennsylvania School of Medicine, Philadelphia (USA)

We have examined the effect of caffeine on the concomitant processes of the repair of potentially lethal damage (PLD) and the synthesis of X-ray-induced proteins in the human malignant melanoma cell line, Ul-Mel. Caffeine administered at a dose of 5mM after X radiation not only inhibited PLD repair but also markedly reduced the level of XIP269, a major X-ray-induced protein whose expression has been shown to correlate with the capacity to repair PLD. The expression of the vast majority of other cellular proteins, including seven other X-ray-induced proteins, remained unchanged following caffeine treatment. A possible role for XIP269 in cell cycle delay following DNA damage by X irradiation is discussed.

• OSTI ID: 5956563
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 125:3; ISSN RAREA; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANALEPTICS
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CAFFEINE
CELL CYCLE
CENTRAL NERVOUS SYSTEM AGENTS
DISEASES
DNA REPAIR
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
MAMMALS
MAN
MELANOMAS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PRIMATES
PROTEINS
PURINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SYNTHESIS
TUMOR CELLS
VERTEBRATES
X RADIATION
XANTHINES