In vivo effect of benzamide and phenobarbital on liver enzymes: poly(adp-ribose) polymerase, cytochrome P-450, styrene oxide hydrolase, cholesterol oxide hydrolase, glutathione S-transferase and udp-glucuronyl transferase

Journal Article · Tue Jul 31 00:00:00 EDT 1984 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/S0006-291X(84)80100-X· OSTI ID:5956341

Griffin, M J; Kirsten, E; Carubelli, R; Palakodety, R B; McLick, J; Kun, E

Rats fed a synthetic diet containing 0.25% benzamide, 0.1% phenobarbital, separately or in combination, for two weeks showed a significant augmentation in the activity of nuclear poly(ADP-ribose) polymerase as well as changes in various nuclear, microsomal and cytosolic liver enzymes involved in the metabolism of xenobiotics. A selective depression of microsomal styrene oxide hydrolase activity by benzamide feeding, and a contrasting augmentation by phenobarbital, were confirmed by immunological titration of the enzyme-protein content suggesting actual enzyme repression and induction. The NAD content of these livers is not altered signficantly as a result of benzamide and phenobarbital feeding, indicating that the changes in enzymes are not a result of non-specific toxic effects. 22 references, 3 tables.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 5956341
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 122:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
BIOLOGICAL EFFECTS
HYDROLASES
ENZYME ACTIVITY
PHENOBARBITAL
POLYMERASES
TRANSFERASES
CYTOCHROMES
IN VIVO
LIVER
METABOLIC ACTIVATION
RATS
ANESTHETICS
ANIMALS
ANTICONVULSANTS
AZINES
BARBITURATES
BODY
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIGESTIVE SYSTEM
DRUGS
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYPNOTICS AND SEDATIVES
MAMMALS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PIGMENTS
PYRIMIDINES
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)