Rapid miniaturized chromatography procedures for iodinated monoclonal antibodies: comparison to gel exclusion chromatography

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · J. Nucl. Med. Technol.; (United States)

OSTI ID:5952645

Kazikiewicz, J M; Zimmer, A M; Spies, S M; Rosen, S T

Chromatographic quality control testing of radioiodinated monoclonal antibodies (/sup 131/I MOAB) is necessary to assess radiochemical purity prior to patient injection. Conventional gel exclusion chromatography column scanning (GCS) is time consuming and not practical. The authors investigated rapid miniaturized chromatographic procedures for evaluating the radiochemical purity of /sup 131/I MOAB. Three systems were evaluated using Gelman ITLC-SG and three solvents: acetone, 85% methanol, and 0.9% NaCl. Radiochemical analysis was performed on Na/sup 131/I of high radiochemical purity and Na/sup 131/I containing radiochemical impurities, as well as three /sup 131/I MOAB preparations. Five separate measurements were obtained for each preparation and solvent, and the results were compared to GCS. The results demonstrated ITLC-SG and 0.9% NaCl was most accurate in assessing radiochemical purity when compared to GCS. With the ITLC-SG and acetone system, and to a lesser degree, the ITLC-SG and 85% methanol system, no separation between /sup 131/I iodate/periodate and /sup 131/I MOAB was achieved, resulting in some instances in the overestimation of the radiochemical purity of the /sup 131/I MOAB.

• Research Organization: Northwestern Memorial Hospital, Chicago, IL
• OSTI ID: 5952645
• Journal Information: J. Nucl. Med. Technol.; (United States), Journal Name: J. Nucl. Med. Technol.; (United States) Vol. 15:3; ISSN JNMTB
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
ALKALI METAL COMPOUNDS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
GEL PERMEATION CHROMATOGRAPHY
HALIDES
HALOGEN COMPOUNDS
IMPURITIES
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 131
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
PHOSPHORS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOCHEMICAL ANALYSIS
RADIOCHROMATOGRAPHY
RADIOISOTOPES
SEPARATION PROCESSES
SODIUM COMPOUNDS
SODIUM IODIDES