Degradation of mono-, di-, and trihalogenated benzoic acids by Pseudomonas aeruginosa JB2
- Univ. of California, Riverside (USA)
Pseudomonas aeruginosa JB2 was isolated from a polychlorinated biphenyl-contaminated soil by enrichment culture containing 2-chlorobenzoate as the sole carbon source. Strain JB2 was subsequently found also to grow on 3-chlorobenzoate, 2,3- and 2,5-dichlorobenzoates, 2,3,5-trichlorobenzoate, and a wide range of other mono- and dihalogenated benzoic acids. Cometabolism of 2,4-dichlorobenzoate was also observed. Chlorocatechols were the central intermediates of all chlorobenzoate catabolic pathways. Degradation of 2-chlorobenzoate was routed through 3-chlorocatechol, whereas 4-chlorocatechol was identified from the metabolism of both 2,3- and 2,5-dichlorobenzoate. The initial attack on chlorobenzoates was oxygen dependent and most likely mediated by dioxygenases. Although plasmids were not detected in strain JB2, spontaneous mutants were detected in 70% of glycerol-grown colonies. The mutants were all of the following phenotype: benzoate{sup +}, 3-chlorobenzoate{sup +}, 2-chlorobenzoate{sup {minus}}, 2,3-dichlorobenzoate{sup {minus}}, 2,5-dichlorobenzoate{sup {minus}}. While chlorocatechols were oxidized by the mutants at wild-type levels, oxidation of 2-chloro- and 2,3- and 2,5-dichlorobenzoates was substantially diminished. These findings suggested that strain JB2 possessed, in addition to the benzoate dioxygenase, a halobenzoate dioxygenase that was necessary for the degradation of chlorobenzoates substituted in the ortho position.
- OSTI ID:
- 5951245
- Journal Information:
- Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:12; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540210* -- Environment
Terrestrial-- Basic Studies-- (1990-)
AROMATICS
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DECOMPOSITION
ENZYMES
HALOGENATED AROMATIC HYDROCARBONS
METABOLISM
MICROORGANISMS
MUTANTS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PHENOTYPE
PSEUDOMONAS