Variation in chlorobenzoate catabolism by Pseudomonas putida P111 as a consequence of genetic alterations

Journal Article · Wed Sep 01 04:00:00 EDT 1993 · Applied and Environmental Microbiology; (United States)

OSTI ID:5809504

Brenner, V; Focht, D D ^[1]; Hernandez, B S ^[2]

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1. Univ. of California, Riverside, CA (United States)
2. Univ. of Panama (Panama)

Chlorobenzoates are key intermediates in the degradative pathways of polychlorinated biphenyls and benzoate herbicides. Bacteria that cometabolize these pollutants generally accumulate chlorobenzoates because they are not able to grow on them. Special interest has been focused on ortho-chlorobenzoates because they are more refractory to biodegradation. In all of these studies the enzyme responsible for the first attack on the ortho-chlorobenzoates possesses minimal or negligible activity with meta- or para-chlorobenzoates. This study reports evidence for the existence of two separate benzoate dioxygenases in Pseudomonas putida P111 and for the transpostional nature of the clc operon, on the basis of genetic investigations of different phenotypic variants of this strain. 42 refs., 4 figs., 1 tab.

• OSTI ID: 5809504
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 59:9; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL ACCUMULATION
BIOLOGICAL PATHWAYS
CATABOLISM
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CONTROL
DECOMPOSITION
GENETIC CONTROL
HALOGENATED AROMATIC HYDROCARBONS
HERBICIDES
METABOLISM
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PEST CONTROL
PESTICIDES
POLLUTANTS
POLYCHLORINATED BIPHENYLS
PSEUDOMONAS