Differential extraction of axonally transported proteoglycans

Journal Article · Mon Oct 01 04:00:00 EDT 1990 · Neurochemical Research; (USA)

DOI:https://doi.org/10.1007/BF00965739· OSTI ID:5946659

Elam, J S ^[1]

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1. Florida State Univ., Tallahassee (USA)

Axonally transported proteoglycans were differentially solubilized by a sequence of extractions designed to infer their relationship to nerve terminal membranes. Groups of goldfish were injected unilaterally with 35SO4 and contralateral optic tecta containing axonally transported molecules were removed 16 h later. Tecta were homogenized in isotonic buffer and centrifuged at 100,000 g for 60 min to create a total supernatant fraction. Subsequent homogenizations followed by recentrifugation were with hypotonic buffer (lysis extract), 1 M NaCl, Triton X-100 or alternatively Triton-1 M NaCl. Populations of proteoglycans in each extract were isolated on DEAE ion exchange columns and evaluated for content of glycosaminoglycans (GAGs). Results show the distribution of transported proteoglycans to be 26.3% total soluble, 13.7% lysis extract, 13.8% NaCl extract, 12.2% Triton extract, and 46.2% Triton-NaCl extract. Proteoglycans from all fractions contained heparan sulfate as the predominant GAG, with lesser amounts of chondroitin (4 or 6) sulfate. The possible localizations of transported proteoglycans suggested by the extraction results are discussed.

• OSTI ID: 5946659
• Journal Information: Neurochemical Research; (USA), Journal Name: Neurochemical Research; (USA) Vol. 15:10; ISSN 0364-3190; ISSN NERED
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CENTRIFUGATION
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GLYCOPROTEINS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
LIGHT NUCLEI
MEMBRANE TRANSPORT
NERVE CELLS
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES
ULTRACENTRIFUGATION