Mechanism of switching from an acidogenic to a butanol-acetone fermentation by Clostridium acetobutylicum. Technical progress report, April 15, 1983-July 1, 1985

Technical Report · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5942203

Rogers, P

We have developed allylalconol resistant mutants that are specifically altered in their product production during fermentation of sugars. Using these mutants of Clostridium acetobutylicum, we have found that they carry specific changes in fermentation enzymes that open the way to studies on the regulatory network by this organism to switch its fermentation pathway. We have determined that synthesis of the butanol producing enzymes is induced just prior to maximum solvent production, resulting in a 20 to 100 fold increase in enzyme specific activity. Our enzymological studies have focused on the two pathways leading from the butyryl-CoA branch point in the fermentation. The conditions for purification and study of the butyrylaldehyde dehydrogenase are in hand. 2 refs., 6 figs., 2 tabs.

• Research Organization: Minnesota Univ., St. Paul (USA)
• DOE Contract Number: AC02-83ER13068
• OSTI ID: 5942203
• Report Number(s): DOE/ER/13068-1; ON: DE86007670
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID
ALCOHOLS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOCONVERSION
BIOLOGICAL PATHWAYS
BUTANOLS
BUTYRIC ACID
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CLOSTRIDIUM
CLOSTRIDIUM ACETOBUTYLICUM
DNA-ASE
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
ESTERASES
FERMENTATION
FRACTIONATION
GAS CHROMATOGRAPHY
GEL PERMEATION CHROMATOGRAPHY
GENE REGULATION
HYDROLASES
HYDROXY COMPOUNDS
KINETICS
METHANOGENIC BACTERIA
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOSPHODIESTERASES
REACTION KINETICS
SEPARATION PROCESSES