Effect of low molecular weight epidermal material upon DNA synthesis in primary cultures of newborn rat keratinocytes

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5942142

Abler, A S

The objective of this study was to isolate inhibitors of replicative DNA synthesis from newborn rat epidermis. The strategy for this study was to assay epidermal extracts for inhibitors of DNA synthesis in primary cultures of newborn rat keratinocytes. DNA synthesis was measured as the incorporation of /sup 4/H-TdR into acid precipitable material. The low molecular weight fraction, LMWF (less than 10Kd), of an aqueous epidermal extract was found to contain activity that inhibits replicative DNA synthesis in primary cultures. The inhibitory activity of the LMWD was detected in a novel assay utilizing primary cultures that were synchronized at the G1/S boundary with the DNA polymerase alpha inhibitor, aphidicolin. LMWF caused a dose dependent inhibition of replicative DNA synthesis as measured by the incorporation of /sup 3/H-TdR into acid precipitable material. The magnitude of the inhibitory effect for a given dose of LMWF was dependent upon the duration of exposure to that dose. The results presented in this investigation suggest that newborn rat epidermis contains a small polypeptide factor that inhibits replicative DNA synthesis in primary culture of newborn rat keratinocytes.

• Research Organization: Michigan Univ., Ann Arbor (USA)
• OSTI ID: 5942142
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BODY
DNA REPLICATION
ENZYME INHIBITORS
EPIDERMIS
EPITHELIUM
HETEROCYCLIC COMPOUNDS
INHIBITION
LABELLED COMPOUNDS
MAMMALS
NEONATES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SKIN
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES