Structure of the Schizosaccharomyces pombe cytochrome c gene
The cytochrome c gene of the fission yeast Schizosaccharomyces pombe has been cloned by using the Saccharomyces cerevisiae iso-1-cytochrome c gene as a molecular hybridization probe. The DNA sequence and the 5' termini of the mRNA transcripts of the gene have been determined. The DNA sequence has confirmed, with two exceptions, the previously determined protein sequence. The nonrandom distribution of silent third base differences which was observed between the two cytochrome c genes of S. cerevisiae does not extend to the S. pombe cytochrome c gene, suggesting that there are no constraints other than protein function and codon usage which have acted to conserve the cytochrome c DNA sequences of the two yeasts. Introduction of the S. pombe cytochrome c gene on a yeast plasmid into a S. cerevisiae mutant which lacked functional cytochrome c transformed that recipient strain for the ability to grow on a nonfermentable carbon source. This implies that the S. pombe cytochrome c gene has all the regulatory signals which are required for its expression in S. cerevisiae, and that none of the amino acid differences between the cytochrome c proteins of the two yeasts has a drastic effect on the function of the protein in vivo.
- Research Organization:
- Dept. of Genetics, Univ. of Washington, Seattle, WA 98195
- OSTI ID:
- 5941887
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 2:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CYTOCHROMES
DNA SEQUENCING
DNA-CLONING
GENE REGULATION
SACCHAROMYCES CEREVISIAE
CELL PROLIFERATION
HYBRIDIZATION
MOLECULAR BIOLOGY
MUTANTS
AMINO ACIDS
BIOSYNTHESIS
CHROMOSOMAL ABERRATIONS
CODONS
CULTURE MEDIA
FERMENTATION
GENES
IN VIVO
MESSENGER-RNA
PLASMIDS
PROTEINS
BIOCONVERSION
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CLONING
FUNGI
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PIGMENTS
PLANTS
RNA
SACCHAROMYCES
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
YEASTS
550400* - Genetics
550200 - Biochemistry