A chilling sensitive mutant of Arabidopsis with altered steryl-ester metabolism

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.93.3.1053· OSTI ID:5941378

Hugly, S; McCourt, P; Somerville, C ^[1]; Browse, J ^[2]; Patterson, G W ^[3]

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1. Michigan State Univ., East Lansing (USA)
2. Washington State Univ., Pullman (USA)
3. Univ. of Maryland, College Park (USA)

A chilling-sensitive mutant of Arabidopsis thaliana was isolated and subjected to genetic, physiological, and biochemical analysis. The chilling-sensitive nature of the mutant line is due to a single recessive nuclear mutation at a locus designated chs1. In contrast to wild-type plants, which are not adversely affected by low temperatures, the chs1 mutant is killed by several days of exposure to temperatures below 18{degree}C. Following exposure to chilling temperatures, the mutant displays two common symptoms of chilling injury - leaf chlorosis and electrolyte leakage. In these respects, the physiological response of the mutant to low temperatures mimics the response observed in some naturally occurring chilling sensitive species. The biochemical basis of chilling sensitivity was explored by examining the pattern of incorporation of {sup 14}CO{sub 2} into soluble metabolites and lipids in wild-type and mutant plants. The only difference observed between the mutant and wild type was that following low temperature treatment, the mutant accumulated 10-fold more radioactivity in a specific class of neutral lipids which were identified by a variety of criteria to be steryl-esters. The accumulation of radioactivity in the steryl-ester fraction occurs 24 hours before there is any visible evidence of chilling injury.

• OSTI ID: 5941378
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 93:3; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL EFFECTS
BODY TEMPERATURE
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHLOROSIS
ELECTROLYTES
GENE MUTATIONS
HERBS
HYDROXY COMPOUNDS
HYPOTHERMIA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
METABOLISM
METABOLITES
MUTANTS
MUTATIONS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PLANTS
SENSITIVITY
STEROIDS
STEROLS
TRACER TECHNIQUES