The aba mutant of Arabidopsis thaliana is impaired in epoxy-carotenoid biosynthesis
- Michigan State Univ., East Lansing (United States)
The three mutant alleles of the ABA locus of Arabidopsis thaliana result in plants that are deficient in the plant growth regulator abscisic acid (ABA). The authors have used {sup 18}O{sub 2} to label ABA in water-stressed leaves of mutant and wild-type Arabidopsis. Analysis by selected ion monitoring and tandem mass spectrometry of ({sup 18}O)ABA and its catabolites, phaseic acid and ABA-glucose ester ({beta}-D-glucopyranosyl abscisate), indicates that the aba genotypes are impaired in ABA biosynthesis and have a small ABA precursor pool of compounds that contain oxygens on the rings, presumably oxygenated carotenoids (xanthophylls). Quantitation of the carotenoids form mutant and wild-type leaves establishes that the aba alleles cause a deficiency of the epoxy-carotenoids violaxanthin and neoxanthin and an accumulation of their biosynthetic precursor, zeaxanthin. These results provide evidence that ABA is synthesized by oxidative cleavage of epoxy-carotenoids (the indirect pathway). Furthermore the carotenoid mutant they describe undergoes normal greening. Thus the aba alleles provide an opportunity to study the physiological roles of epoxy-carotenoids in photosynthesis in a higher plants.
- OSTI ID:
- 5634244
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:17; ISSN PNASA; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSCISIC ACID
ARABIDOPSIS
AUXINS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CAROTENOIDS
CHEMICAL REACTIONS
EVEN-EVEN NUCLEI
GENOTYPE
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METABOLISM
MONOCARBOXYLIC ACIDS
MUTANTS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN 18
OXYGEN ISOTOPES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PIGMENTS
PLANT GROWTH REGULATORS
PLANTS
STABLE ISOTOPES
SYNTHESIS
TERPENES
TRACER TECHNIQUES