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Title: Xanthophylls and abscisic acid biosynthesis in water-stressed bean leaves

Abstract

Experiments were designed to obtain evidence about the possible role of xanthophylls as abscisic acid (ABA) precursors in water-stressed leaves of Phaseolus vularis L. Leaves were exposed to /sup 14/CO/sub 2/ and the specific activities of several major leaf xanthophylls and stress-induced ABA were determined after a chase in /sup 12/CO/sub 2/ for varying periods of time. The ABA specific radioactivities were about 30 to 70% of that of lutein and violaxanthin regardless of the chase period. The specific activity of neoxanthin, however, was only about 15% of that of ABA. The effects of fluridone on xanthophyll and ABA levels and the extent of labeling of both from /sup 14/CO/sub 2/ were determined. Fluridone did not inhibit the accumulation of ABA when leaves were stressed once, although subsequent stresses in the presence of fluridone did lead to a reduced ABA accumulation. The incorporation of /sup 14/C from /sup 14/CO/sub 2/ into ABA and the xanthophylls was inhibited by fluridone and to about the same extent. The incorporation of /sup 18/O into ABA from violaxanthin which had been labeled in situ by means of the violaxanthin cycle was measured. The results indicated that a portion of the ABA accumulated during stressmore » was formed from violaxanthin which had been labeled with /sup 18/O. The results of these experiments are consistent with a preformed xanthophyll(s) as the major ABA precursor in water-stressed bean leaves.« less

Authors:
;
Publication Date:
Research Org.:
State Univ. of New York, Syracuse
OSTI Identifier:
5640424
Resource Type:
Journal Article
Resource Relation:
Journal Name: Plant Physiol.; (United States); Journal Volume: 85:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ABSCISIC ACID; BIOSYNTHESIS; PHASEOLUS; BIOLOGICAL STRESS; PIGMENTS; CARBON 14 COMPOUNDS; CARBON DIOXIDE; LEAVES; OXYGEN 18; PRECURSOR; RESPONSE MODIFYING FACTORS; TRACER TECHNIQUES; WATER; AUXINS; BACTERIA; CARBON COMPOUNDS; CARBON OXIDES; CARBOXYLIC ACIDS; CHALCOGENIDES; EVEN-EVEN NUCLEI; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; LEGUMINOSAE; LIGHT NUCLEI; MICROORGANISMS; MONOCARBOXYLIC ACIDS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; OXYGEN ISOTOPES; PLANT GROWTH REGULATORS; PLANTS; RHIZOBIUM; STABLE ISOTOPES; SYNTHESIS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Li, Y., and Walton, D.C. Xanthophylls and abscisic acid biosynthesis in water-stressed bean leaves. United States: N. p., 1987. Web. doi:10.1104/pp.85.4.910.
Li, Y., & Walton, D.C. Xanthophylls and abscisic acid biosynthesis in water-stressed bean leaves. United States. doi:10.1104/pp.85.4.910.
Li, Y., and Walton, D.C. Tue . "Xanthophylls and abscisic acid biosynthesis in water-stressed bean leaves". United States. doi:10.1104/pp.85.4.910.
@article{osti_5640424,
title = {Xanthophylls and abscisic acid biosynthesis in water-stressed bean leaves},
author = {Li, Y. and Walton, D.C.},
abstractNote = {Experiments were designed to obtain evidence about the possible role of xanthophylls as abscisic acid (ABA) precursors in water-stressed leaves of Phaseolus vularis L. Leaves were exposed to /sup 14/CO/sub 2/ and the specific activities of several major leaf xanthophylls and stress-induced ABA were determined after a chase in /sup 12/CO/sub 2/ for varying periods of time. The ABA specific radioactivities were about 30 to 70% of that of lutein and violaxanthin regardless of the chase period. The specific activity of neoxanthin, however, was only about 15% of that of ABA. The effects of fluridone on xanthophyll and ABA levels and the extent of labeling of both from /sup 14/CO/sub 2/ were determined. Fluridone did not inhibit the accumulation of ABA when leaves were stressed once, although subsequent stresses in the presence of fluridone did lead to a reduced ABA accumulation. The incorporation of /sup 14/C from /sup 14/CO/sub 2/ into ABA and the xanthophylls was inhibited by fluridone and to about the same extent. The incorporation of /sup 18/O into ABA from violaxanthin which had been labeled in situ by means of the violaxanthin cycle was measured. The results indicated that a portion of the ABA accumulated during stress was formed from violaxanthin which had been labeled with /sup 18/O. The results of these experiments are consistent with a preformed xanthophyll(s) as the major ABA precursor in water-stressed bean leaves.},
doi = {10.1104/pp.85.4.910},
journal = {Plant Physiol.; (United States)},
number = ,
volume = 85:4,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 1987},
month = {Tue Dec 01 00:00:00 EST 1987}
}