Branched chain amino acid metabolism in the biosynthesis of Lycopersicon pennellii glucose esters

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.93.4.1544· OSTI ID:5941320

Walters, D S; Steffens, J C ^[1]

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1. Cornell Univ., Ithaca, NY (USA)

Lycopersicon pennellii Corr. (D'Arcy) an insect-resistant, wild tomato possesses high densities of glandular trichomes which exude a mixture of 2,3,4-tri-O-acylated glucose esters that function as a physical impediment and feeding deterrent to small arthropod pests. The acyl moieties are branched C{sub 4} and C{sub 5} acids, and branched and straight chain C{sub 10}, C{sub 11}, and C{sub 12} acids. The structure of the branched acyl constituents suggests that the branched chain amino acid biosynthetic pathway participates in their biosynthesis. ({sup 14}C)Valine and deuterated branched chain amino acids (and their oxo-acid derivatives) were incorporated into branched C{sub 4} and C{sub 5} acid groups of glucose esters by a process of transamination, oxidative decarboxylation and subsequent acylation. C{sub 4} and C{sub 5} branched acids were elongated by two carbon units to produce the branched C{sub 10}-C{sub 12} groups. Norvaline, norleucine, allylglycine, and methionine also were processed into acyl moieties and secreted from the trichomes as glucose esters. Changes in the acyl composition of the glucose esters following sulfonylurea herbicide administration support the participation of acetohydroxyacid synthetase and the other enzymes of branched amino acid biosynthesis in the production of glucose esters.

• OSTI ID: 5941320
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 93:4; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
ALDEHYDES
AMINO ACIDS
ANIMALS
ARTHROPODS
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECARBOXYLATION
ESTERS
FOOD
FRUITS
GLUCOSE
HEXOSES
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHYSIOLOGY
SACCHARIDES
TOLERANCE
TOMATOES
TRACER TECHNIQUES