System for the exposure of cell suspensions to power-frequency electric fields
A system is described that uses an oscillating magnetic field to produce power-frequency electric fields with strengths in excess of those produced in an animal or human standing under a high-voltage electric-power transmission line. In contrast to other types of exposure systems capable of generating fields of this size, no electrodes are placed in the conducting growth media: the possibility of electrode contamination of the exposed suspension is thereby eliminated. Electric fields in the range 0.02-3.5 V/m can be produced in a cell culture with total harmonic distortions less than 1.5%. The magnetic field used to produce electric fields for exposure is largely confined within a closed ferromagnetic circuit, and experimental and control cells are exposed to leakage magnetic flux densities less than 5 ..mu..T. The temperatures of the experimental and control cell suspensions are held fixed within +/-0.1 /sup 0/C by a water bath. Special chambers were developed to hold cell cultures during exposure and sham exposure. Chinese hamster ovary (CHO) cells incubated in these chambers grew for at least 48 h and had population doubling times of 16-17 h, approximately the same as for CHO cells grown under standard cell-culture conditions. 17 references, 6 figures, 2 tables.
- Research Organization:
- Pacific Northwest Lab., Richland, WA
- OSTI ID:
- 5940570
- Journal Information:
- Bioelectromagnetics (N.Y.); (United States), Journal Name: Bioelectromagnetics (N.Y.); (United States) Vol. 5; ISSN BLCTD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
CELL CULTURES
CHO CELLS
DATA
DESIGN
ELECTRIC FIELDS
EXPERIMENTAL DATA
EXPOSURE CHAMBERS
GROWTH
INFORMATION
MAGNETIC FIELDS
NUMERICAL DATA