Brain receptor autoradiography with ( sup 3 H)-YM 09151-2: A ligand for labeling dopamine D-2 receptors

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Life Sciences; (USA)

OSTI ID:5935489

Unis, A S; Vincent, J G; Dillon, B ^[1]

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1. Univ. of Washington, Seattle (USA)

Using the technique of in vitro receptor autoradioagraphy to slide-mounted tissue sections, the authors studied the suitability of ({sup 3}H)-YM-09151-2 as a ligand for labeling D-2 receptors in adult F344 rat brains. Specific ({sup 3}H)-YM-09151-2 binding accounted for 70-80% of the total bound ligand and reached equilibrium after a 60-90 minute incubation. Scatchard analysis revealed a K{sub d} of 626 pM. The apparent B{sub max} was 23.2 fmol/tissue section. Autoradiographs demonstrated high grain densities in the striatum and olfactory tubercle. Diffuse specific binding was also observed in the cortex.

• OSTI ID: 5935489
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 47:25; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
DOPAMINE
DRUGS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LABELLING
LIGANDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SYMPATHOMIMETICS
TRITIUM COMPOUNDS
VERTEBRATES