Presence in photosystem II core complexes of a 34-kilodalton polypeptide required for water photolysis
Journal Article
·
· Plant Physiol.; (United States)
Photosystem II (PSII) reaction center core complexes have been isolated and characterized from wild type (WT) Scenedesmus obliquus and from its LF-1 mutant. LF-1 thylakoids are blocked on the oxidizing side of PSII and have a reduced Mn content. Visible absorption and low temperature fluorescence spectra of both core complexes are identical and resemble those reported for spinach. Lithium dodecyl sulfate-polycrylamide gel electrophoresis reveals that a protein alteration, originally observed in thylakoid membranes is retained in the PSII core particles. That is, a 34-kilodalton (kD) polypeptide, present in the WT core complex, is missing in the mutant, and the core complex of the mutant contains a 36-kD protein not present in the WT. The 34-kD intrinsic protein is also observed in O/sub 2/-evolving PSII preparations and PSII core complexes from spinach. It is distinct from the 33-kD extrinsic protein first reported by T. Kuwabara and N. Murata. We suggest that the 34-kD protein is a site of Mn binding in the PSII membrane. 21 references, 3 figures.
- Research Organization:
- Solar Energy Research Inst., Golden, CO
- DOE Contract Number:
- AC02-83CH10093
- OSTI ID:
- 5934809
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 76:3; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Identification of a 34 kDa protein altered in the LF-1 mutant as the herbicide-binding D1 protein of photosystem II
Phosphorylation of photosystem II components, CP43 apoprotein, D1, D2, and 10 to 11 kilodalton protein in chloroplast thylakoids of higher plants
Changes in protein composition and Mn abundance in photosystem II particles on photoactivation of the latent O/sub 2/-evolving system in flash-grown wheat leaves. [Triticum aestivum L]
Conference
·
Mon Mar 31 23:00:00 EST 1986
· Plant Physiol.; (United States)
·
OSTI ID:5383198
Phosphorylation of photosystem II components, CP43 apoprotein, D1, D2, and 10 to 11 kilodalton protein in chloroplast thylakoids of higher plants
Journal Article
·
Sat Oct 31 23:00:00 EST 1987
· Plant Physiol.; (United States)
·
OSTI ID:5563213
Changes in protein composition and Mn abundance in photosystem II particles on photoactivation of the latent O/sub 2/-evolving system in flash-grown wheat leaves. [Triticum aestivum L]
Journal Article
·
Tue Dec 31 23:00:00 EST 1985
· Plant Physiol.; (United States)
·
OSTI ID:5531457
Related Subjects
14 SOLAR ENERGY
140505 -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOCONVERSION
BIOPHOTOLYSIS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL BONDS
CHEMICAL REACTIONS
DECOMPOSITION
ELECTROPHORESIS
ELEMENTS
FLUORESCENCE
HYDROGEN COMPOUNDS
LUMINESCENCE
MANGANESE
MEMBRANES
METALS
MICROORGANISMS
MUTANTS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDES
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PHOTOSYNTHESIS
PHOTOSYNTHETIC REACTION CENTERS
PLANTS
POLYPEPTIDES
PROTEINS
SCENEDESMUS
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSITION ELEMENTS
UNICELLULAR ALGAE
WATER
140505 -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOCONVERSION
BIOPHOTOLYSIS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL BONDS
CHEMICAL REACTIONS
DECOMPOSITION
ELECTROPHORESIS
ELEMENTS
FLUORESCENCE
HYDROGEN COMPOUNDS
LUMINESCENCE
MANGANESE
MEMBRANES
METALS
MICROORGANISMS
MUTANTS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDES
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PHOTOSYNTHESIS
PHOTOSYNTHETIC REACTION CENTERS
PLANTS
POLYPEPTIDES
PROTEINS
SCENEDESMUS
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSITION ELEMENTS
UNICELLULAR ALGAE
WATER