Changes in protein composition and Mn abundance in photosystem II particles on photoactivation of the latent O/sub 2/-evolving system in flash-grown wheat leaves. [Triticum aestivum L]
Protein composition and Mn abundance were compared between the two photosystem II (PSII) particle preparations obtained before and after photoactivation of the latent O/sub 2/-evolving system in intermittently flashed wheat leaves. The following results have been obtained: (a) nonphotoactivated PSII particles were devoid of two extrinsic proteins which corresponded to the 24 and 16 kilodalton proteins in spinach particles, although the particles contained all the intrinsic proteins and the 33 kilodalton extrinsic protein. (b) The two extrinsic proteins absent in nonphotoactivated PSII particles were present in nonphotoactivated thylakoids, but were easily removed by a hypotonic shock followed by brief sonication. Such removal of the proteins did not occur in photoactivated thylakoids. (c) Nonphotoactivated PSII particles contained 1.5 Mn/400 chlorophyll, while photoactivated particles contained 8 Mn/400 chlorophyll. (d) Nonphotoactivated thylakoids contained 6 Mn/400 chlorophyll, but most of them were removed from thylakoids by a hypotonic shock in the presence of ethylenediaminetetraacetate. Such removal of Mn did not occur in photoactivated thylakoids.
- Research Organization:
- Institute of Physical and Chemical Research (RIKEN), Saitama, Japan
- OSTI ID:
- 5531457
- Journal Information:
- Plant Physiol.; (United States), Vol. 80:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Structure of the manganese complex of photosystem II upon removal of the 33-kilodalton extrinsic protein: an X-ray absorption spectroscopy study
Phosphorylation of photosystem II components, CP43 apoprotein, D1, D2, and 10 to 11 kilodalton protein in chloroplast thylakoids of higher plants
Related Subjects
PHOTOSYNTHETIC REACTION CENTERS
PROTEINS
CHEMICAL COMPOSITION
VISIBLE RADIATION
BIOLOGICAL EFFECTS
WHEAT
BIOLOGICAL SHOCK
CHLOROPHYLL
EDTA
EXPERIMENTAL DATA
LEAVES
MANGANESE
OXYGEN
QUANTITY RATIO
REMOVAL
SPINACH
AMINO ACIDS
CARBOXYLIC ACIDS
CEREALS
CHELATING AGENTS
DATA
ELECTROMAGNETIC RADIATION
ELEMENTS
FOOD
GRASS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFORMATION
METALS
NONMETALS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
RADIATIONS
TRANSITION ELEMENTS
VEGETABLES
550500* - Metabolism