16,16-Dimethyl prostaglandin E2 increases survival in mice following irradiation

Technical Report · Thu Jan 01 04:00:00 UTC 1987

OSTI ID:5934212

Walden, T L; Patchen, M; Snyder, S L

16,16-Dimethyl prostaglandin E2(DiPGE2), a stable analog of PGE2, increases the LD 50/30 survival in CD2F1 male mice when given prior to ionizing radiation. Subcutaneous administration of 40 microgram of DiPGE2 30 min prior to /sup 60/Co gamma irradiation extends the LD 50/30 from 9.39 Gy in the control animals to 16.14 Gy in DiPGE2 treated, with a dose-reduction factor of 1.72p95% confidence limits: 1.62, 1.82. The degree of protection is dependent on both the time of administration and the dose of the prostaglandin. Ten micrograms administered 5 min prior to receiving a lethal dose of 10 Gy provides 90% survival but only 10% survival if administered 30 min prior to irradiation. Experiments to determine the in vivo concentration of DiPGE2 in organs post injection show increased levels over time, but these are not correlated with protection. At 30 min after injection, as much as 80% of the DiPGE2 present in the spleen and plasma is unmetabolized. These results suggest that the protection results from the physiologic action of DiPGE2 rather than direct in vivo detoxification of radicals.

• Research Organization: Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
• OSTI ID: 5934212
• Report Number(s): AD-A-183500/8/XAB; AFRRI-SR-87-21
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYL RADICALS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
DETOXIFICATION
DOSES
DRUGS
EFFICIENCY
IRRADIATION
LETHAL DOSES
LETHAL RADIATION DOSE
MAMMALS
METHYL RADICALS
MICE
ORGANS
PROSTAGLANDINS
RADIATION DOSES
RADIATION EFFECTS
RADICALS
RADIOPROTECTIVE SUBSTANCES
RODENTS
SPLEEN
VERTEBRATES