GroEL and dnaK genes of Escherichia coli are induced by UV irradiation and nalidixic acid in an htpR/sup +/-dependent fashion
Two proteins with molecular weights of 61,000 and 73,000 were found to be induced by UV light in Escherichia coli mutants in which the SOS responses are constitutively expressed. The induction of these proteins by UV light and nalidixic acid was shown to be independent of the recA/sup +/ lexA/sup +/ regulatory system. Analysis of these proteins by two-dimensional gel electrophoresis and comparison with the heat-shock proteins of E. coli revealed that the M/sub r/ 61,000 protein comigrated with the groEL gene product, that the M/sub r/ 73,000 protein comigrated with the dnaK gene product, and that other heat-shock proteins were also induced. The induction of groEL and dnaK by UV light and nalidixic acid is controlled by the htpR locus. The results suggest that the regulatory response of E. coli to agents such as UV light and nalidixic acid is more complex than previously thought. 35 references, 6 figures, 1 table.
- Research Organization:
- Massachusetts Inst. of Tech., Cambridge
- OSTI ID:
- 5929260
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 81:5; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ESCHERICHIA COLI
GENES
GENETIC EFFECTS
MICROORGANISMS
MUTANTS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
STRUCTURAL CHEMICAL ANALYSIS
TEMPERATURE EFFECTS
ULTRAVIOLET RADIATION