groE mutants of Escherichia coli are defective in umuDC-dependent UV mutagenesis

Journal Article · Wed Nov 01 04:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:5023012

Donnelly, C E; Walker, G C ^[1]

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1. Massachusetts Institute of Technology, Cambridge (USA)

Overexpression of the SOS-inducible umuDC operon of Escherichia coli results in the inability of these cells to grow at 30 degrees C. Mutations in several heat shock genes suppress this cold sensitivity. Suppression of umuD+C+-dependent cold sensitivity appears to occur by two different mechanisms. We show that mutations in lon and dnaK heat shock genes suppress cold sensitivity in a lexA-dependent manner. In contrast, mutations in groES, groEL, and rpoH heat shock genes suppress cold sensitivity regardless of the transcriptional regulation of the umuDC genes. We have also found that mutations in groES and groEL genes are defective in umuDC-dependent UV mutagenesis. This defect can be suppressed by increased expression of the umuDC operon. The mechanism by which groE mutations affect umuDC gene product function may be related to the stability of the UmuC protein, since the half-life of this protein is shortened because of mutations at the groE locus.

• OSTI ID: 5023012
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:11; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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BIOLOGICAL HALF-LIFE
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
DNA REPAIR
ELECTROMAGNETIC RADIATION
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GENE MUTATIONS
GENE OPERONS
GENE REGULATION
GENOTYPE
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ORGANIC COMPOUNDS
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RADIOINDUCTION
RECOVERY
REPAIR
TEMPERATURE EFFECTS
ULTRAVIOLET RADIATION