groE mutants of Escherichia coli are defective in umuDC-dependent UV mutagenesis

Journal Article · Wed Nov 01 00:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:5023012

Donnelly, C E; Walker, G C ^[1]

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1. Massachusetts Institute of Technology, Cambridge (USA)

Overexpression of the SOS-inducible umuDC operon of Escherichia coli results in the inability of these cells to grow at 30 degrees C. Mutations in several heat shock genes suppress this cold sensitivity. Suppression of umuD+C+-dependent cold sensitivity appears to occur by two different mechanisms. We show that mutations in lon and dnaK heat shock genes suppress cold sensitivity in a lexA-dependent manner. In contrast, mutations in groES, groEL, and rpoH heat shock genes suppress cold sensitivity regardless of the transcriptional regulation of the umuDC genes. We have also found that mutations in groES and groEL genes are defective in umuDC-dependent UV mutagenesis. This defect can be suppressed by increased expression of the umuDC operon. The mechanism by which groE mutations affect umuDC gene product function may be related to the stability of the UmuC protein, since the half-life of this protein is shortened because of mutations at the groE locus.

• OSTI ID: 5023012
• Journal Information: Journal of Bacteriology; (USA), Vol. 171:11; ISSN 0021-9193
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ESCHERICHIA COLI
DNA REPAIR
GENE MUTATIONS
RADIOINDUCTION
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BIOLOGICAL HALF-LIFE
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560130* - Radiation Effects on Microorganisms