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Effect of protein diet on age-dependent methylation of liver nuclear components of mice administered N-nitrosodimethylamine

Journal Article · · J. Natl. Cancer Inst.; (United States)
OSTI ID:5928054
;

Protein diets deficient or enriched in DL-methionine were studied for the effect of methylation of nuclear components in subadult and adult outbred albino male mice given N-nitrosodimethylamine. After the mice were fed diets for 6 days, they were given ip injections of (/sup 14/C)N-nitrosodimethylamine (5 mg/kg body wt) and killed 45 minutes later. Liver nuclei were isolated; incorporation of /sup 14/C into nuclear proteins and methylation of DNA and RNA were determined. In the subadult and adult animals the nuclear protein content was diminished by shortage of a single essential amino acid. Incorporation of /sup 14/C into the total protein was reduced. The reduction amounted to 55% in the subadult and 23% in the adult animals. Neither an age-dependent nor a diet-dependent change in the specific methylation of DNA and RNA was observed. Analysis of methylation of DNA and RNA was observed. Analysis of methylated purine bases showed an age-related rise in O6-methylguanine in the adult as compared with the subadult mice.

Research Organization:
Univ. of Stockholm, Sweden
OSTI ID:
5928054
Journal Information:
J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 73:4; ISSN JNCIA
Country of Publication:
United States
Language:
English

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Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
AMINES
AMINO ACIDS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DIET
DIGESTIVE SYSTEM
DNA
GLANDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
METHYLATION
MICE
NITROSAMINES
NITROSO COMPOUNDS
NUCLEIC ACIDS
NUTRITIONAL DEFICIENCY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
RESPONSE MODIFYING FACTORS
RNA
RODENTS
TRACER TECHNIQUES
VERTEBRATES