Effect of protein diet on age-dependent methylation of liver nuclear components of mice administered N-nitrosodimethylamine
Protein diets deficient or enriched in DL-methionine were studied for the effect of methylation of nuclear components in subadult and adult outbred albino male mice given N-nitrosodimethylamine. After the mice were fed diets for 6 days, they were given ip injections of (/sup 14/C)N-nitrosodimethylamine (5 mg/kg body wt) and killed 45 minutes later. Liver nuclei were isolated; incorporation of /sup 14/C into nuclear proteins and methylation of DNA and RNA were determined. In the subadult and adult animals the nuclear protein content was diminished by shortage of a single essential amino acid. Incorporation of /sup 14/C into the total protein was reduced. The reduction amounted to 55% in the subadult and 23% in the adult animals. Neither an age-dependent nor a diet-dependent change in the specific methylation of DNA and RNA was observed. Analysis of methylation of DNA and RNA was observed. Analysis of methylated purine bases showed an age-related rise in O6-methylguanine in the adult as compared with the subadult mice.
- Research Organization:
- Univ. of Stockholm, Sweden
- OSTI ID:
- 5928054
- Journal Information:
- J. Natl. Cancer Inst.; (United States), Vol. 73:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
METHYLATION
NITROSAMINES
BIOLOGICAL EFFECTS
RNA
AGE DEPENDENCE
AMINO ACIDS
CARBON 14 COMPOUNDS
DIET
LIVER
MICE
NUTRITIONAL DEFICIENCY
PROTEINS
RESPONSE MODIFYING FACTORS
TRACER TECHNIQUES
AMINES
ANIMALS
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
GLANDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)